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rabbit polyclonal anti caspase 8 p43 p18  (Proteintech)


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    Proteintech rabbit polyclonal anti caspase 8 p43 p18
    Rabbit Polyclonal Anti Caspase 8 P43 P18, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+caspase+8/Caspase+8%2Fp43%2Fp18+Antibody/pmc12866116-27-0-6
    Average 96 stars, based on 395 article reviews
    rabbit polyclonal anti caspase 8 p43 p18 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome.
    Article Snippet: The antibodies used in this study were as follows: rabbit monoclonal anti-S100A9 antibody (1:800, 73425, CST, USA), mouse monoclonal anti-TLR4 antibody (1:1000, MA5-16216, Thermo Fisher, USA), mouse monoclonal anti-MyD88 antibody (1:1000, 67969-1-Ig, Proteintech, China), rabbit polyclonal anti-P38 (1:1000, B-IO-10130, Biozellen, USA), rabbit polyclonal p-p38 (1:800, B-IO10131, Biozellen, USA), rabbit monoclonal anti-NF-κB (1:1000, 8242, CST, USA), rabbit monoclonal anti-pNF-κB (1:800, 3033, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), rabbit monoclonal anti-IκBα antibody (1:1000, 2859, CST, USA), mouse monoclonal anti-IL-1β (1:800, sc-12742, Santa Cruz Biotechnology, USA), mouse monoclonal anti-IL-6 (1:500, sc-57315, Santa Cruz Biotechnology, USA), TNF-α (1:1000, 17590-1-AP, Proteintech, China), rabbit polyclonal anti-TNF-α (1:600, 17590-1-AP, Proteintech, China), mouse monoclonal anti-GAPDH (1:1000, 60004-1-Ig, Proteintech, China), mouse monoclonal anti-caspase-3 (1:600, 66470-2-Ig, Proteintech, China), rabbit polyclonal anti-caspase-8 (1:600, 13423-1-AP, Proteintech, China), rabbit monoclonal anti-caspase-1 antibody (1:800, 24232, CST, USA), mouse monoclonal anti-Bax (1:1000, 60267-1-Ig, Proteintech, China), mouse monoclonal anti-UPK3A (1:800, sc-166808, Santa Cruz Biotechnology, USA), rabbit polyclonal anti-UPK2 (1:600, 21149-1-AP, Proteintech, China), goat anti-rabbit secondary antibody (1:2000, G6120, Thermo Fisher, USA) and goat anti-mouse secondary antibody (1:2000, G21040, Thermo Fisher, USA).

    Article Title: S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome
    Article Snippet: The antibodies used in this study were as follows: rabbit monoclonal anti-S100A9 antibody (1:800, 73425, CST, USA), mouse monoclonal anti-TLR4 antibody (1:1000, MA5-16216, Thermo Fisher, USA), mouse monoclonal anti-MyD88 antibody (1:1000, 67969-1-Ig, Proteintech, China), rabbit polyclonal anti-P38 (1:1000, B-IO-10130, Biozellen, USA), rabbit polyclonal p-p38 (1:800, B-IO-10131, Biozellen, USA), rabbit monoclonal anti-NF-κB (1:1000, 8242, CST, USA), rabbit monoclonal anti-p-NF-κB (1:800, 3033, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), rabbit monoclonal anti-IκBα antibody (1:1000, 2859, CST, USA), mouse monoclonal anti-IL-1β (1:800, sc-12742, Santa Cruz Biotechnology, USA), mouse monoclonal anti-IL-6 (1:500, sc-57315, Santa Cruz Biotechnology, USA), TNF-α (1:1000, 17590-1-AP, Proteintech, China), rabbit polyclonal anti-TNF-α (1:600, 17590-1-AP, Proteintech, China), mouse monoclonal anti-GAPDH (1:1000, 60004-1-Ig, Proteintech, China), mouse monoclonal anti-caspase-3 (1:600, 66470-2-Ig, Proteintech, China), rabbit polyclonal anti-caspase-8 (1:600, 13423-1-AP, Proteintech, China), rabbit monoclonal anti-caspase-1 antibody (1:800, 24232, CST, USA), mouse monoclonal anti-Bax (1:1000, 60267-1-Ig, Proteintech, China), mouse monoclonal anti-UPK3A (1:800, sc-166808, Santa Cruz Biotechnology, USA), rabbit polyclonal anti-UPK2 (1:600, 21149-1-AP, Proteintech, China), goat anti-rabbit secondary antibody (1:2000, G6120, Thermo Fisher, USA) and goat anti-mouse secondary antibody (1:2000, G21040 , Thermo Fisher, USA).

    Activation Assay:

    Article Title: Lactobacillus rhamnosus Ameliorates Multi-Drug-Resistant Bacillus cereus -Induced Cell Damage through Inhibition of NLRP3 Inflammasomes and Apoptosis in Bovine Endometritis
    Article Snippet: .. The following primary antibodies were used: rabbit polyclonal anti-NLRP3 (1:1000 dilution, 19771-1-AP), rabbit polyclonal anti-ASC (also known as apoptosis-associated speck-like protein containing a caspase activation and recruitment domain; 1:500 dilution, 10500-1-AP) (ProteinTech Group, Rosemont, IL, USA), rabbit polyclonal anti-Occludin (1:2000 dilution, ab216327) (Abcam, Boston, MA, USA), mouse monoclonal anti-ZO-1 (1:1000 dilution, 66452-1-Ig), rabbit polyclonal anti-BAX (1:4000 dilution, 50599-Ig), rabbit polyclonal anti-Bcl-2 (1:1000 dilution, 12789-1-AP), rabbit polyclonal anti-PARP (1:1000, 13371-1-AP), mouse monoclonal anti-caspase-3 (1:1000 dilution, 66470-2-Ig), rabbit polyclonal anti-caspase 8 (1:1000 dilution, 13423-1-AP), rabbit polyclonal anti-caspase 9 (1:1000 dilution, 10380-1-AP) (ProteinTech Group, Rosemont, IL, USA), rabbit polyclonal anti-FAS (a pro-apoptotic TNF receptor (TNFR) superfamily member) (1:500, WL03376), rabbit polyclonal anti-SMAC (the second mitochondria-derived activator of caspase) (1:500, WL0834), and rabbit polyclonal anti-TNF-R1 (1:500, WL01414) (Wanlaibio, Shenyang, China). ..

    Article Title: Lactobacillus rhamnosus Ameliorates Multi-Drug-Resistant Bacillus cereus -Induced Cell Damage through Inhibition of NLRP3 Inflammasomes and Apoptosis in Bovine Endometritis.
    Article Snippet: .. The following primary antibodies were used: rabbit polyclonal anti-NLRP3 (1:1000 dilution, 19771-1-AP), rabbit polyclonal anti-ASC (also known as apoptosis-associated speck-like protein containing a caspase activation and recruitment domain; 1:500 dilution, 10500-1-AP) (ProteinTech Group, Rosemont, IL, USA), rabbit polyclonal anti-Occludin (1:2000 dilution, ab216327) (Abcam, Boston, MA, USA), mouse monoclonal anti-ZO-1 (1:1000 dilution, 66452-1-Ig), rabbit polyclonal anti-BAX (1:4000 dilution, 50599-Ig), rabbit polyclonal anti-Bcl-2 (1:1000 dilution, 12789-1-AP), rabbit polyclonal anti-PARP (1:1000, 13371-1-AP), mouse monoclonal anti-caspase-3 (1:1000 dilution, 66470-2-Ig), rabbit polyclonal anti-caspase 8 (1:1000 dilution, 13423-1-AP), rabbit polyclonal anti-caspase 9 (1:1000 dilution, 10380-1-AP) (ProteinTech Group, Rosemont, IL, USA), rabbit polyclonal anti-FAS (a pro-apoptotic TNF receptor (TNFR) superfamily member) (1:500, WL03376), rabbit polyclonal anti-SMAC (the second mitochondria-derived activator of caspase) (1:500, WL0834), and rabbit polyclonal antiTNF-R1 (1:500, WL01414) (Wanlaibio, Shenyang, China). ..

    Western Blot:

    Article Title: HSP110 Inhibition in Primary Effusion Lymphoma Cells: One Molecule, Many Pro-Survival Targets
    Article Snippet: After three further washes, the membranes were finally subjected to ECL (Advansta, San Jose, CA, USA, cat n. 12045-D20). .. The following primary antibodies were used in Western blots: Rabbit polyclonal anti-HSP110 (1:200) (Abcam, Cambridge UK, cat. n. ab24503), rabbit polyclonal anti-PARP (1:1000) (Cell Signaling, Danvers, MA, USA, cat n. 9542), rabbit polyclonal anti-phospho STAT3 Tyr705 (1:500) (Cell Signalling Danvers, MA, USA, cat n. 9145), mouse monoclonal anti-STAT3 (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat n. sc-482), mouse monoclonal anti-BRCA-1 (1:1000) (EMD Millipore, Burlington, MA, USA, cat n. OP92), mouse monoclonal anti-pH2AX (Ser 139) (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat n. sc-517348), mouse monoclonal anti-KU70 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat n. sc-17789), rabbit polyclonal anti-Caspase-8 (1:200) (Proteintech, Manchester UK, cat. n. 13423-1-AP), rabbit polyclonal anti-BID (1:200) (Proteintech, Manchester UK, cat. n. 10988-1-AP), mouse monoclonal anti-p21 (1:500) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, cat n. sc-397), and rabbit polyclonal anti-c-Myc (1:200) (Proteintech, Manchester UK, cat. n. 10828-1-AP). .. Mouse monoclonal anti-b-Actin (1:10,000) (Sigma-Aldrich, St. Louis, MO, USA cat n. A5441) was used to detect the loading control.



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    Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for <t>CASP8,</t> CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
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    Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for <t>CASP8,</t> CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
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    Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD

    Journal: BMC Molecular and Cell Biology

    Article Title: Alcohol exposure induces ferroptosis-dominated programmed cell death in esophageal epithelial cells

    doi: 10.1186/s12860-026-00589-5

    Figure Lengend Snippet: Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD

    Article Snippet: The following primary antibodies were used: Beclin-1 (Origene, #TA502643), PCNA, CASP1 (Abcam, #ab179515), CASP3 (Santa Cruz Biotechnology, #sc-271759), CASP8 (Origene, #TA374288), CASP9 (Origene, #TA4227045), endoG, GPX4 (Origene, #TA423164M), GSDMD, IL-1β (Santa Cruz Biotechnology, #sc-12742), MLKL, phosphor-MLKL (Abcam, #ab196436), SLC7A11 (Origene, #TA423232), LC3B, BAX, GAPDH (OriGene, #TA800894), and β-actin (Santa Cruz Biotechnology, Inc. #sc-69879).

    Techniques: Incubation, Control, Western Blot, Expressing, Staining, Translocation Assay, CCK-8 Assay